Yes, design your layout in photoshop, then slice it up the way you want it. At the bottom of the tools bar in photoshop you will see a little icon to jump to imageready,this will copy your image to imageready. Once in imageread, select the "Slice Selection Tool", click on a slice, and type in its link. Do this for all the images. Then, go to File>>Save As>> and select "html and images". Save it in a new folder anywhere you want, the html code will be saved and the images saved inside a seperate folder.
Upload it to your server, or if you have a localserver setup, view it there :)